Multiple players from Essendon and one player from one other AFL club are being investigated for possible performance enhancing drug use.
AFL deputy chief executive Gillon McLachlan addressed the media in Melbourne on Sunday and said the Australian Crime Commission report only identified two specific cases where WADA prohibited performance enhancing drugs may have been used in the AFL.
McLachlan confirmed one of those clubs was Essendon - who went to the AFL with concerns over supplement use before the ACC handed down their report on Thursday.
McLachlan said the possible breaches of the AFL Doping code would be investigated.
'The AFL is aware of one case involving the possibility of WADA prohibited performance enhancing drug use by one player at one club,' said McLachlan, before saying he was not in a position to name that club.
'The AFL is aware that a second case involves the possibility of WADA prohibited performance enhancing drug use by multiple players at one club.
'In this case it's possible that players were administered the WADA prohibited substances without their knowledge or consent.'
McLachlan said the AFL was not aware of any specific instances of suspected match fixing in AFL competition.