Hawthorn utility Cyril Rioli has aggravated a leg injury during the AFL pre-season.
Rioli was already ruled out of the indigenous all-stars team that will travel to Alice Springs for Friday night's exhibition match against Richmond.
On Monday, Rioli aggravated a corked quad injury that he has been nursing for the past week.
All-stars captain and Hawks teammate Lance Franklin said Rioli had wanted to play in Friday's match.
'He's a little bit sore at the moment,' Franklin said.
'He's pretty disappointed - with me being the captain, he wanted to run out with me.'
Shaun Burgoyne is another Hawthorn player ruled out of the all-stars team as he continues his rehabilitation from off-season ankle surgery.
But teammates Bradley Hill, Amos Frank and Jed Anderson are in the squad.
Burgoyne, Sydney co-captain Adam Goodes and former Fremantle defender Roger Hayden will be assistants to all-stars coach Michael O'Loughlin.
'The side that's going to be out there (will) be a very exciting list,' Franklin said.
'(It's) a little bit disappointing Goodesy's not playing and Shauny Burgoyne and Cyril.
'But the young boys we have running around out there can play some football.'
Richmond will also miss some experienced players, Nathan Foley still recovering from last year's achilles tendon injury and former Port Adelaide defender Troy Chaplin also yet to reach match fitness.
'There will be a good mix of senior players, but also we will give an opportunity to some young players,' said new Tigers captain Trent Cotchin.
'He (Chaplin) just started training back with the main group after having post-season surgery.
'He will probably miss this game, but he's definitely on the right track to play some senior footy in the near future.'
Cotchin added that Foley was back running and making steady progress.
'He's very important to the way we play and we saw when he is in the team, what sort of impact he can have.
'It will be nice to have him back - it will be like having a new draftee.'